Confocal and two photon microscopy foundations applications and advances pdf

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confocal and two photon microscopy foundations applications and advances pdf

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Skip to search form Skip to main content You are currently offline. Some features of the site may not work correctly. Diaspro Published Chemistry.

Confocal and two‐photon microscopy: Foundations, applications and advances

Giberto Giuseppe Chirico Physics Dept. The Journal of cell biology 7 , , Journal of Physics D: Applied Physics 48 44 , , Biochemical and biophysical research communications 1 , , Journal of Photochemistry and Photobiology B: Biology 55 1 , , Journal of Biological Chemistry 18 , ,

Confocal and two-photon microscopy : foundations, applications, and advances

Protocol DOI: Vertebrate vision is maintained by the retinoid visual cycle, a complex enzymatic pathway that operates in the retina to regenerate the visual chromophore, cis -retinal, a prosthetic group of rhodopsin that undergoes activation by light. Many different mutations in genes encoding retinoid cycle proteins can cause a variety of human blinding diseases. Two-photon microscopy is an evolving, non-invasive, and repetitive imaging technology that can be used to monitor biomolecules within the vertebrate retina at a subcellular resolution. This method has the great advantage of portraying endogenous retinoid fluorophores in their native state without the need for artificial staining. Such real-time retinal imaging permits rapid evaluation not only of various stages of retinal disease in live animal models of human retinopathies but also of the outcome from intended pharmacological therapies. Two-photon microscopy offers substantial potential for early detection of age- and disease-related changes in the eye, long before clinical or pathological manifestations become apparent.

Skip to search form Skip to main content You are currently offline. Some features of the site may not work correctly. DOI: Description: Confocal and Two—Photon Microscopy Foundations, Applications, and Advances Edited by Alberto Diaspro Confocal and two—photon fluorescence microscopy has provided researchers with unique possibilities of three—dimensional imaging of biological cells and tissues and of other structures such as semiconductor integrated circuits.

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