Tipp city schools ch 18 pearson physics interference and diffraction pdf

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Colocalization analysis in fluorescence micrographs: verification of a more accurate calculation of pearson 's correlation coefficient. One of the most routine uses of fluorescence microscopy is colocalization, i. Frequently this is presented simplistically by the use of overlays of red and green images, with areas of yellow indicating colocalization of the molecules. Colocalization data are rarely quantified and can be misleading. Our results from both synthetic and biological datasets demonstrate that the generation of Pearson 's correlation coefficient between pairs of images can overestimate positive correlation and fail to demonstrate negative correlation.

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